期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 34, 期 8, 页码 2110-2118出版社
WILEY
DOI: 10.1002/eji.200424958
关键词
human; intestine; T lymphocytes; NKT cells; tumor immunity
类别
T cells expressing INK cell receptors (NKR) display rapid MHC-unrestricted cytotoxicity and potent cytokine secretion and are thought to play roles in immunity against tumors. We have quantified and characterized NKR+ T cells freshly isolated from epithelial and lamina propria layers of duodenum and colon from 16 individuals with no evidence of gastrointestinal disease and from tumor and uninvolved tissue from 19 patients with colorectal cancer. NKR+ T cell subpopulations were differentially distributed in different intestinal compartments, and CD161(+) T cells accounted for over one half of T cells at all locations tested. Most intestinal CD161(+)T cells expressed alpha beta TCR and either CD4 or CD8. Significant proportions expressed HLA-DR, CD69 and Fas ligand. Upon stimulation in vitro, CD161(+) T cells produced IFN-gamma and TNF-alpha but not IL-4. NKT cells expressing the V alpha 24V beta 11 TCR, which recognizes CD1d, were virtually absent from the intestine, but colonic cells produced IFN-gamma in response to the NKT cell agonist ligand alpha-galactosylceramide. NKR+ T cells were not expanded in colonic tumors compared to adjacent uninvolved tissue. The predominance, heterogeneity and differential distribution of NKR+ T cells at different intestinal locations suggests that they are central to intestinal immunity.
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