期刊
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
卷 36, 期 8, 页码 1502-1509出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2003.12.001
关键词
A(3)AR; IB-MECA; MRS1220; RT-PCR; cAMP; breast cancer cell lines
A(3) adenosine receptor agonists have been reported to influence cell death and survival. Here we report the effects of an A(3) adenosine receptor agonist, IB-MECA, on the cell growth of human breast cancer cell lines, MCF-7 (estrogen receptor positive) and MDA-MB468 (estrogen receptor negative). Therefore, this study was aimed to investigate the expression and possible action of A(3) receptor in the human breast cancer cell lines. IB-MECA, at 1-100 muM, resulted in a significant cell growth inhibition (P < 0.05) which reached the maximum at 48 h, in the cell lines. In both cell lines, agonist-induced effects were antagonized by pretreatment with a selective A(3) adenosine receptor antagonist, MRS1220. Using RT-PCR method, further confirmation was provided by the presence of mRNA of A(3) receptor in the cells. In addition, IB-MECA was able to inhibit forskolin-stimulated cAMP levels, which indicate the functional form of A(3) receptor on the cell surface of these breast cancer cell lines. These results suggest that the inhibitory effect of IB-MECA on the growth of human breast cancer cell lines is mediated through activation of A(3) adenosine receptor. (C) 2004 Elsevier Ltd. All rights reserved.
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