期刊
JOURNAL OF MICROSCOPY-OXFORD
卷 215, 期 -, 页码 139-148出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.0022-2720.2004.01362.x
关键词
brain; contrast agent; microtomography; microvascular; sample preparation; synchrotron radiation; X-ray
类别
This paper presents the first application of high-resolution X-ray synchrotron tomography to the imaging of large microvascular networks in biological tissue samples. This technique offers the opportunity of analysing the full three-dimensional vascular network from the micrometre to the millimetre scale. This paper presents the specific sample preparation method and the X-ray imaging procedure. Either barium or iron was injected as contrast agent in the vascular network. The impact of the composition and concentration of the injected solution on the X-ray synchrotron tomography images has been studied. Two imaging modes, attenuation and phase contrast, are compared. Synchrotron high-resolution computed tomography offers new prospects in the three-dimensional imaging of in situ biological vascular networks.
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