期刊
MOLECULAR BREEDING
卷 14, 期 1, 页码 73-82出版社
SPRINGER
DOI: 10.1023/B:MOLB.0000037996.01494.12
关键词
bean alpha-amylase inhibitor 1 (alpha AI1); Callosobruchus spp.; chickpea transformation; Cicer arietinum; transgenic chickpeas; weevil resistance
We describe a robust and reproducible Agrobacterium-mediated chickpea transformation method based on kanamycin selection, and its use to introduce the bean alphaAI1 gene into a desi type of chickpea. Bean alphaAI1 was specifically expressed in the seeds, accumulated up to 4.2% of seed protein and was processed to low molecular weight polypeptides as occurs in bean seeds. The transgenic protein was active as an inhibitor of porcine alpha-amylase in vitro. Transgenic chickpeas containing alpha-AI1 strongly inhibited the development of Callosobruchus maculatus and C. chinensis (Col. : Bruchidae) in insect bioassays.
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