期刊
BIOTECHNOLOGY LETTERS
卷 26, 期 16, 页码 1265-1268出版社
SPRINGER
DOI: 10.1023/B:BILE.0000044870.99039.19
关键词
alpha-L-rhamnosidase; Aspergillus terreus; beta-D-glucosidase; dye affinity chromatography; naringinase complex
Affinity chromatography with immobilised triazine dyes was used to separate the main enzymes present in the naringinase complex produced by Aspergillus terreus CECT 2663. One alpha-L-rhamnosidase and two beta-D-glucosidases (betaG1 and betaG2) were separated by a simple two-step procedure involving chromatography with Red HF-3B immobilised on Sepharose 4B first at pH 5.5 and then at pH 4.7. Maximum activity of the beta-D-glucosidases was from pH 4 to 6 and at 65 degreesC. Both glucosidases were active on p-nitrophenol glucoside and prunin with respective Km values of 1.9 mM and 1.6 mM for betaG1 and 2.1 mM and 0.25 mM for betaG2. Only betaG1 hydrolysed cellobiose (Km = 5.7 mM).
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