期刊
APPLIED SPECTROSCOPY
卷 65, 期 11, 页码 1250-1253出版社
SAGE PUBLICATIONS INC
DOI: 10.1366/11-06357
关键词
Interaction; Bovine hemoglobin; BPA; Ultraviolet-visible spectroscopy; Circular dichroism; Molecular modeling
资金
- NSFC [20875055]
- Ministry of Education of China [708058]
- Key Science-Technology Project in Shandong Province [2008GG10006012]
The interaction of bisphenol A with bovine hemoglobin (BHb) under physiological conditions was investigated by using fluorescence, ultraviolet-visible (UV-Vis) absorption, circular dichroism (CD), and molecular modeling. The experimental results showed that BPA can bind with BHb to form a complex. The binding constant Ka and the number of binding sites n were calculated to be 1.49 x 10(5) L mol(-1) and 1, respectively. Molecular modeling study revealed that BPA bound into BHb central cavity, and the binding mode of BPA-BHb complex could be hydrogen bonding. The UV-Vis absorption and CD spectra indicated that the secondary structure of BHb was altered, which may affect physiological functions of hemoglobin. This work is helpful for clarifying the molecular toxic mechanism of BPA in vivo.
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