期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 70B, 期 2, 页码 223-232出版社
WILEY-LISS
DOI: 10.1002/jbm.b.30044
关键词
FTIR-ATR; vascular graft; healing; polytetrafluoroethylene; polyester; fibronectin; growth factors
A bigraft, composed of a 30-mum internodal distance expanded polytetrafluoroethylene (ePTFE) arterial graft segment and a preclotted polyethylene terephthalate arterial graft segment, was used to study the healing process of two different materials in the same dog. Healing was followed by FTIR-ATR spectroscopy and correlated with ELISA analyses of selected growth factors and matrix proteins. The FTIR analyses of the ePTFE explants from 1 and 3 h; 3, 5, 7, 10, and 14 days; and 4 and 8 weeks showed that the ePTFE grafts did not heat (endothelialize), but appeared to remain in overlapping inflammation and proliferation stages of wound healing. Although FN was found in the fibrin layer coating the luminal surface of the ePTFE, VEGF was not present. The inability of VEGF to complex with FN may be the result of FN binding sites being blocked or hidden by the conformation of the fibrin clot that forms on ePTFE. Also, TGF-beta was not present beyond the initial clot formation at 3 h postimplantation. The absence of these two growth factors may be an important factor in the lack of healing of 30-mum ePTFE grafts, because both were found to be present during the early stages of healing for the preclotted polyester grafts that did endothelialize. (C) 2004 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据