期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 101, 期 34, 页码 12700-12705出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0405065101
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资金
- NIAAA NIH HHS [P50 AA012870, AA 13430, K02 AA013430, AA 12870] Funding Source: Medline
- NIBIB NIH HHS [EB 002097, R01 EB002097] Funding Source: Medline
- NICHD NIH HHS [HD 32573, P01 HD032573] Funding Source: Medline
- NIDDK NIH HHS [DK 27121, R01 DK027121] Funding Source: Medline
- NINDS NIH HHS [NS 34813, R01 NS034813] Funding Source: Medline
Multivolume H-1-[C-13] NMR spectroscopy in combination with i.v. [1,6-C-13(2)]glucose infusion was used to detect regional glucose metabolism and glutamatergic neurotransmission in the halo-thane-anesthetized rat brain at 7 T. The regional information was decomposed into pure cerebral gray matter, white matter, and subcortical structures by means of tissue segmentation based on quantitative T, relaxation mapping. The 13C turnover curves of [4-C-13]glutamate, [4-C-13]glutamine, and [3-C-13]glutamate + glutamine were fitted with a two-compartment neuronal-astroglial metabolic model. The neuronal tricarboxylic acid cycle fluxes in cerebral gray matter, white matter, and subcortex were 0.79 +/- 0.15, 0.20 +/- 0.11, and 0.42 +/- 0.09 mumol/min per g, respectively. The glutamate-glutamine neurotransmitter cycle fluxes in cerebral gray matter, white matter, and subcortex were 0.31 +/- 0.07, 0.02 +/- 0.04, and 0.18 +/- 0.12 mumol/min per g, respectively. The exchange rate between the mitochondrial and cytosolic metabolite pools was fast relative to the neuronal tricarboxylic acid cycle flux for all cerebral tissue types.
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