期刊
MOLECULAR ECOLOGY NOTES
卷 4, 期 3, 页码 446-448出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.1471-8286.2004.00676.x
关键词
cross priming; dinucleotide repeats; enriched library; microsatellite; pea aphid
Microsatellite loci were isolated from enriched partial genomic libraries of Acyrthosiphon loti and Acyrthosiphon pisum. Twenty of those loci were characterized in A. pisum. Fifteen of those loci were polymorphic. Genetic diversity varied across loci, allele repeat number ranging from two to 15, and observed heterozygosity from 0.1 and 0.96. An additional eight microsatellite loci originally isolated from other aphids but cross-priming with A. pisum showed polymorphism as well. Allele size ranged from three to 9 and observed heterozygosity from 0.43 to 0.84. Overall, we present 23 microsatellite loci that can be used to reveal polymorphism in pea aphids.
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