Effect of platelet-released growth factors and collagen type I on osseous regeneration of mandibular defects -: A pilot study in minipigs

Objectives: To study the effects of platelet-released growth factors (PRGF) and collagen type I on bone defect healing in minipig mandibles. Material and Methods: In eight adult minipigs defects were trephined in the facial mandibular wall from extra-oral and filled with collagen+PRGF or with collagen alone. Control defects were left untreated. PRGF were defined as the supernatants obtained after centrifugation of washed, thrombin-activated allogenic cells of platelet-rich plasma. The animals were sacrificed at 4 and 8 weeks. For histological analysis, undecalcified ground specimens stained with the Levai-Laczko stain were used. Results: For the entire follow-up, the amount of newly formed bone was 35.49+/-3.84% in the collagen+PRGF group, 46.34+/-3.84% in the collagen-only group and 33.83+/-4.11% in the controls. The differences between the collagen+PRGF and the collagen-only group (p=0.0343), and between the collagen-only group and the controls (p=0.0305) were significant. Histologically, defects filled with collagen+PRGF showed inflammatory reactions at 4 weeks, and new bone formation near the remnants of the filler collagen was reduced. Conclusion: The data suggest that collagen type I alone, but not its combination with PRGF can support the early stages of cortical bone repair.