期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 42, 期 9, 页码 703-708出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2004.07.003
关键词
carbon dioxide; carbon economy; CO2 refixation; developing oilseeds; rapeseed; soybean
A new method has been developed to rapidly determine the total inorganic carbon concentration (gaseous [CO2] + aqueous [CO2] + [HCO3-] + [CO32-]) in developing seeds. Seeds are rapidly dissected and homogenized in 1 N HCl in gas-tight vials. The headspace gas is then analyzed by infrared gas analysis. Developing rapeseed (Brassica napus L.) and soybean [Glycine max (L.) Merr.] seeds were analyzed and found to have up to 40 and 12 mM total inorganic carbon, respectively. These concentrations are ca. 600-2000-fold higher than in ambient air or values reported for leaves. Carbon dioxide concentrations in rapeseed peaked during the stage of maximum oil synthesis and declined as seeds matured. The consequences for seed metabolism, physiology and carbon economy are discussed. (C) 2004 Elsevier SAS. All rights reserved.
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