期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷 139, 期 1, 页码 103-109出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2004.07.001
关键词
cAMP; Chasmagnathus granulatus; crab; dopamine; gills; ion transport; Na+/K+-ATPase; PKA; transepithelial potential differences
Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10parts per thousand salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte), which reflects ion transport activity. Dopamine (DA) also had a stimulatory effect on ion uptake, increasing Vte and Na+ influx, although this effect was transient, since both variables remained elevated for less than 30 min. In addition, the dose-response curve for DA concentration-Vte was biphasic, and the maximum stimulation was obtained with 10 mumol l(-1). When the effects of forskolin and DA on the Na+/K+-ATPase activity were tested, they correlated well with the Vte and Na+ influx experiments; the enzyme activity increased significantly after preincubation of gill fragments for 10 min with forskolin or DA (51 and 64%, respectively), but there was no effect after pre-incubation with DA for 20 min. Finally, KT5720, a specific inhibitor of cAMP-dependent protein kinase (PKA), completely abolished the stimulatory effect of DA on Vte, suggesting the involvement of PKA in this mechanism. (C) 2004 Elsevier Inc. All rights reserved.
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