期刊
MARINE BIOTECHNOLOGY
卷 6, 期 5, 页码 485-492出版社
SPRINGER
DOI: 10.1007/s10126-004-3121-2
关键词
human coagulation factor VII; transgenic fish; bioreactor
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVII was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
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