期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 3, 期 9, 页码 857-871出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M400045-MCP200
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资金
- NCRR NIH HHS [S10 RR017990-01] Funding Source: Medline
- NIA NIH HHS [R01 AG13620] Funding Source: Medline
- NINDS NIH HHS [T32 NS 07457-04, R21 NS44184-01] Funding Source: Medline
The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.
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