The effect of bacterial motion on the diffusion of a molecule of high molecular weight is studied by observing the mixing of two streams of fluid in a microfluidic flow cell. We show that the presence of motile E. coli bacteria in one of the streams results in a marked increase in the effective diffusion coefficient of Dextran, which rises linearly with the concentration of bacteria from a baseline value of 0.2x10(-7) to 0.8x10(-7) (cm(2)/s) at a concentration of 2.1x10(9)/ml (approximately 0.5% by volume). Furthermore, we observe that the diffusion process is also observed to undergo a change from standard Fickian diffusion to a superdiffusive behavior in which the diffusion exponent rises from 0.5 to 0.55 as the concentration of bacteria rises from 0 to 2.1x10(9)/ml. (C) 2004 American Institute of Physics.
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