期刊
APPLIED RADIATION AND ISOTOPES
卷 67, 期 7-8, 页码 S321-S324出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.apradiso.2009.03.071
关键词
Photodynamic therapy; Boron neutron capture therapy; Porphyrin; Melanotic melanoma
A water-soluble [meso-tetra(4-nido-carboranylphenyl)porphyrin] (H2TCP) bearing 36 boron atoms was studied for its accumulation and its radio/photo-sensitization efficiency towards murine melanotic melanoma cells. The amount of H2TCP in the cells increased with the porphyrin dose in the incubation medium up to 100 mu M with no significant dark toxicity. Fluorescence microscopy observations showed that the porphyrin was largely localized intracellularly. Based on these in vitro results our investigations were pursued using the B16F1 melanotic melanoma subcutaneously transplanted in C57BL6 mice as in vivo model. Phormacokinetic studies were performed by injection of H2TCP intratumorally (1 mg/kg) and intravenously (10 mg/kg). At 0.5 h after is. administration or at 24 h after i.v. injection, the amounts of B-10 in the turnout were about 60 ppm and about 6 ppm, respectively. The distribution of H2TCP in the turnout after intravenous or intratumoural injection was also assessed by fluorescence microscopy analyses. Under these conditions, preliminary BNCT studies were carried out using a new thermal column called HYTOR (HYbrid Thermal spectrum sHifter TapirO Reactor) inserted in the fast nuclear reactor Tapiro at Enea Casaccia, Italy. The mice were exposed to HYTHOR radiation field for 20 min at a reactor power of 5 kW. In spite of different amounts of B-10 in the turnout at the irradiation time, a similar significant delay in tumour growth (5-6 days) was induced by neutron irradiation in intratourmorally and intravenously injected mice. The response of the melanotic melanoma to H2TCP-BNCT was compared with that obtained by irradiation after intraperitoneal injection of boron-phenylalanine. (C) 2009 Elsevier Ltd. All rights reserved.
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