期刊
MUSCLE & NERVE
卷 30, 期 3, 页码 310-316出版社
JOHN WILEY & SONS INC
DOI: 10.1002/mus.20098
关键词
intraepidermal nerve fibers; neurofilaments; neuropathy; PGP 9.5; skin biopsy; tubules
The cytoplasmic protein gene product 9.5 (PGP 9.5) is considered a reliable marker for intraepidermal nerve fibers (IENFs). However, PGIP 9.5 expression has never been compared with antibodies against the main components of the cytoskeleton. We compared the density of PGP 9.5-positive IENF at the leg with that obtained using a panel of antibodies specific for certain cytoskeletal components, namely, anti-unique P-tubulin (TuJ1), anti-nonphosphorylated microtubule-associated protein-1B ((MAP1B), anti-70 and 200 KDa neurofilament (NF), and anti phosphorylated neurofilament (SMI 312), in 15 healthy subjects and in 10 patients with painful neuropathy. We also performed colocalization studies and investigated the relationship between IENFs and Schwann cells. In both controls and neuropathies, the density of IENF labeled by PGP 9.5, TuJ1, and MAP1B did not differ, whereas that of NF and SMI 312 was significantly lower. Double-staining studies confirmed that antibodies against cytoskeletal markers can be used to reliably stain skin nerve fibers, suggesting that they might provide insight into specific axonal impairment in peripheral neuropathies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据