期刊
ARCHIVES OF ORAL BIOLOGY
卷 49, 期 9, 页码 739-746出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2004.04.004
关键词
cyclooxygenase-2; lipopolysaccharide; immunohistochemistry; periodontitis; rat model
Up-regulation of prostaglandin E-2 (PGE(2)) production in the periodontal tissue is considered to be important for periodontal tissue destruction. The purpose of the study was to demonstrate the dynamic changes of immuno-localization of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in rat periodontal tissue after topical. application of lipopolysaccharide (LPS: 5 mg/ml. in physiological saline) from Escherichia coli into the rat molar gingival sulcus. In the normal periodontal tissue, small numbers of junctional epithelium (JE) cells and numerous osteocytes embedded in alveolar bone constitutively expressed COX-1. The COX-1 expression was not effected by LPS application. JE cells, especially in the coronal. portion of JE also expressed COX-2. LPS application induced the JE cells with consequent transient expression of COX-2 with a peak at day 1. These findings suggest that JE cells may play a critical role in first defense tine against LIDS challenge and PGE(2) from JE cells may be responsible for the initiation of periodontal inflammation. In the deep periodontal tissue, cementoblasts and osteoblasts showed constitutive expression of COX-2, which may be induced by continuous cyclic tension force due to occlusal. pressure. LIPS application caused a transient up-regulation of COX-2 expression in periodontal. ligament fibroblasts, cementoblasts and osteoblasts. It is suggested that the inducible production of PGE(2) via COX-2 by these cells may be associated with connective tissue destruction and alveolar bone resorption. (C) 2004 Elsevier Ltd. All rights reserved.
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