期刊
STRUCTURE
卷 12, 期 9, 页码 1595-1605出版社
CELL PRESS
DOI: 10.1016/j.str.2004.06.018
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资金
- NIAID NIH HHS [N01 AI-75320] Funding Source: Medline
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 Angstrom resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii.
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