期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 68, 期 9, 页码 2001-2006出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.68.2001
关键词
biosynthesis; diterpene cyclase; momilactone; phytoalexin; rice
Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. Using rice genome information and in vitro assay with recombinant enzymes, we identified genes (OsKS4 and OsKS10) encoding the type-A diterpene cyclases 9beta-pimara-7,15-diene synthase and ent-sandaracopimara-diene synthase which are involved in the biosynthesis of momilactones A, B and oryzalexins A-F respectively. Transcript levels of these two genes increased remarkably after ultraviolet (UV) treatment, which is consistent with elevated production of phytoalexins by UV. These two genes might prove powerful tools for understanding plant defense mechanisms in rice.
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