期刊
INFECTION AND IMMUNITY
卷 72, 期 9, 页码 4948-4955出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.72.9.4948-4955.2004
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资金
- NCRR NIH HHS [G12 RR003037, RR 03037] Funding Source: Medline
- NIAID NIH HHS [R01 AI19990, R01 AI019990] Funding Source: Medline
- NIGMS NIH HHS [S06 GM060654] Funding Source: Medline
- PHS HHS [S06 60654] Funding Source: Medline
Candida albicans maintains both commensal and pathogenic states in humans. Both states are dependent on cell surface-expressed adhesins, including those of the A1s family. Heterologous expression of A1s5p at the surface of Saccharomyces cerevisiae results in A1s5p-mediated adhesion to various ligands, followed by formation of multicellular aggregates. Following adhesion of one region of the cell to fibronectin-coated beads, the entire surface of the cells became competent to mediate cell-cell aggregation. Aggregates formed in the presence of metabolic inhibitors or signal transduction inhibitors but were reduced in the presence of 8-anilino-1-naphthalene-sulfonic acid (ANS) or Congo Red (CR), perturbants that inhibit protein structural transitions. These perturbants also inhibited aggregation of C. albicans. An increase in ANS fluorescence, which accompanied Als-dependent cellular adhesion, indicated an increase in cell surface hydrophobicity. In addition, C. albicans and A1s5p-expressing S. cerevisiae showed an aggregation-induced birefringence indicative of order on the cell surface. The increase in birefringence did not occur in the presence of the aggregation disruptants ANS and CR. These results suggest a model for A1s5p-mediated aggregation in which an adhesion-triggered change in the conformation of A1s5p propagates around the cell surface, forming ordered aggregation-competent regions.
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