期刊
EXPERIMENTAL LUNG RESEARCH
卷 30, 期 6, 页码 479-493出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/01902140490476382
关键词
direct visualization; inhalation; intratracheal instillation; isotope recovery; lung; lung injury; pulmonary edema
资金
- NHLBI NIH HHS [HL-51854] Funding Source: Medline
Although several methods have been used to deliver fluid, into the distal airspaces of the lung, the efficiency of these methods has been variable. Therefore, the authors have modified prior techniques to design a better method for direct visual instillation (DVI) of fluid into the trachea and compared its efficiency with two commonly used methods: nasal inhalation and invasive intratracheal instillation (delivery of the instillate by needle puncture of the trachea). The results showed that this method (DVI) can deliver fluid efficiently into either both lungs or into a single lung. Using all. I-131-albumin labeling technique, DVI resulted in 92 +/- 1% retention of the labeled albumin in the lungs 1 hour after instillation, significantly greater than nasal inhalation (48 +/- 3%, P <. 01) and invasive intratracheal instillation (77 +/- 3%, P <.05). Also, when bacteria (Escherichia coli) were instilled, with the DVI method, the severity of gram-negative pneumonia, was greater (65 +/- 0.5 g water/g dry weight) compared to delivery by nasal inhalation (5.5 +/- 0.4 g water/g dry weight, P<.05) or by invasive intratracheal instillation (5.9 +/- 0.4 g water/g dry weight, P <.05). The authors conclude that DVI is more efficient, than nasal inhalation and invasive intratracheal instillation for delivering experimental fluids into the distal airspaces of anesthetized mice. This method should. be valuable for experimental lung studies in mice.
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