期刊
ANALYTICAL BIOCHEMISTRY
卷 332, 期 1, 页码 116-121出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.05.052
关键词
peptides; tyrosine; phosphorylation; Raman; src
Drop-coating-deposition-Raman (DCDR) is used to detect spectral changes induced by phosphorylation of tyrosine amino acid residues in peptides. Four peptides are investigated, with sequences derived from the human protein-tyrosine kinase, p60c-src, with Y-216, Y-419, and Y-530 phosphorylation sites. Although the spectra of the four peptides are quite different, tyrosine phosphorylation is found to invariably induce the collapse of a doublet at 820-850 cm(-1) and the attenuation of a peak around 1205 cm(-1). Moreover, amide III band shifts suggest that tyrosine phosphorylation may promote beta sheet formation., particularly in peptides that lack phenylalanine residues. The degree of tyrosine phosphorylation in peptide mixtures is determined using DCDR combined with partial least squares multivariate calibration with a 2%, root mean standard error of prediction. (C) 2004 Elsevier Inc. All rights reserved.
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