期刊
JOURNAL OF BIOMOLECULAR SCREENING
卷 9, 期 6, 页码 498-505出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057104267605
关键词
affinity selection; mass spectrometry; high-throughput screening; orphan targets; nontractable targets; chemical genomics
SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.
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