期刊
AQUACULTURE
卷 238, 期 1-4, 页码 155-164出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2004.05.020
关键词
tetraploids; Megalobrama amblycephala; heat shock; fertility
Using thermal induction, fertile tetraploids of Megalobrama amblycephala were generated. Survival of hatchlings, from eggs that were heat shocked at different time intervals post fertilization, showed that the eggs were sensitive at two different ages after fertilization and the first one was more suitable for thermal induction than the latter. A thermal shock at 40 degreesC for 2 min applied 33 min (tau(0):1.40) after fertilization to eggs resulted in the highest yield (6.3%) of tetraploids. At the age of 330 days, tetraploidy was confirmed in them by karyotyping and erythrocyte nuclear volume measurement by particle size analysis. Induction at the optimum thermal shock to 51-min (tau(0):2.17) old fertilized eggs led not only to poor yield (1.7%) but also to significantly higher frequency (32%) of abnormal juveniles. Male tetraploids attained sexual maturity at the age of 2(+), when only 11.4% of the 4n females were matured. The remaining 71.4% of females matured at age of 3(+). Hatching success of the cross 4nfemale x 4nmale was 60.8% with mean tetraploidy rate of 93.2% in the offspring. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据