期刊
VACCINE
卷 22, 期 25-26, 页码 3243-3255出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2004.05.014
关键词
typhimurium; Salmonella pathogenicity Island-2; ssaG promoter; in vivo-inducible expression; chromosomal expression; E. coli heat labile toxin
DNA derived from regions upstream of the Salmonella enterica serovar Typhimurium ssaG gene were used to drive expression of different reporter genes in putative Salmonella vaccine strains. Expression from ssaG was shown to be significantly upregulated once Salmonella had entered murine or human macrophages, and levels of expression were dependent on the length of the ssaG 5' sequence incorporated. S. Typhimurium derivatives harbouring the Escherichia coli heat labile toxin B subunit (LT-B) fused to various lengths of the ssaG promoter region were also constructed as single copy chromosomal integrations. Expression of LT-B by these Salmonella derivatives was detected at significant levels after intra-macrophage survival and mice immunised with these derivatives mounted marked anti-LT-B Immoral antibody responses. (C) 2004 Elsevier Ltd. All rights reserved.
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