Ingestion by deposit-feeding macro-zoobenthos in the aphotic zone does not affect the pool of live pelagic diatoms in the sediment

The ability of deposit feeders to utilise the pool of live planktonic diatoms in-the sediment was investigated after the spring bloom in 2001 and 2002 at four sediment sites in the aphotic zone in the Kattegat. Seven species of deposit-feeding bivalves, gastropods and polychaetes were allowed to defecate in containers with filtered seawater. A total of 22 containers were set up holding between 3 and 32 animals, grouped by station and taxa. When defecation was completed, the animals were reintroduced to different stocks of homogenised sediment which were manipulated by addition of luminophores and spores of the diatom Chaetoceros diadema, and the faeces again collected and analysed. Our analysis included the number of germinable planktonic diatoms, measured by the dilution extinction method, and the concentrations of the algae pigments fucoxanthin and chl a, in the faeces samples, in the sediment stocks and in the sediment at the sampling location. Comparison between the sediment composition at the sampling locations, and the composition of the corresponding faeces, showed that the concentration of germinable diatoms averaged about 50,000 g(-1) dry weight (DW) in the surface sediment as well as in the faeces in 2001, while in 2002, the average concentration was about 390,000 g(-1) DW in both faeces and in the sediment. A similar comparison in the second part of the experiments also showed that the diatoms in the sediment were unaffected by gut passage and the added spores of C. diadema germinated in the same quantity in the faeces as in the sediment. The taxonomic composition of the diatoms in the faeces matched the composition at the respective stations and sediment stocks. Finally, the concentration of pigments did not change significantly during gut passage. The biomass of live planktonic diatoms in the area of study was estimated to be 2 - 10 g DW/m(2) or 0.2-5% of the total organic content in the top 3 cm of the sediment. It is concluded that this pool of diatoms is largely unaffected by deposit feeders and seems to be unimportant as a food source. It is hypothesised that the input of the spring bloom should be considered as composed of two fractions playing different roles for the benthic ecosystem. One fraction of dead organic material may be utilised immediately, while the fraction of live planktonic diatoms serve as a stable food buffer, which gradually become available to deposit feeders after the diatoms die and degradation starts. (C) 2004 Elsevier B.V. All rights reserved.