期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 429, 期 2, 页码 154-163出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2004.06.019
关键词
Maillard reaction products; monosaccharides; antioxidant activity; hydroxyl radicals; DPPH; H2O2; AAPH; transition metal ions; caco-2 cell; cytotoxicity
Model Maillard reaction (MR) products (MRPs) employing lysine with an aldohexose (e.g., glucose), ketohexose (e.g., fructose), and aldopentose (e.g., ribose) sugars were generated (e.g., pH 9.0; over 2 h heating at 120degrees) and fractionated with ethanol into low (LMW) and high (HMW) molecular weight fractions. Characteristically different temporal patterns of fluorescence and ultraviolet/ visible absorption spectra were obtained from the three distinct sugar-lysine MRPs, and corresponded to different yields of total and dialyzable carbon, indicating that relative reaction rates and degree of polymerization favored the Rib-Lys MRP, compared to GluLys and Fru-Lys MRPs, respectively (p < 0.05). Further characterization of antioxidant activity of the sugar specific-lysine MRPs in chemical (e.g., hydrophobic (1,1,-diphenyl-2-picryl-hydrazyI radical (DPPH) and hydrophilic (Fenton reaction-induced hydroxyl radical) in vitro scavenging assays showed that Rib-Lys HMW M RPs had the highest (p <0.05) affinity to scavenge free radicals. All sugar-Lys MRPs, however, displayed similar protection of cultured Caco-2 cells from exposure to H2O2-, 2,2, -azobis-(2-amidinopropane) dihydrochloride (AAPH)-, ferrous (Fe2+)-, and cupric (cu(2+))-induced cytotoxicity, evaluated both from redox (e.g., MTT response) and cell membrane integrity (e.g., LDH secretion). HMW-MRPs exhibited stronger (p < 0.05) antioxidant activity to scavenge hydroxyl and DPPH radicals, and a greater (p < 0.05) protective effect against both Fe2+- and Cu2 (+)-induced cytotoxicity in Caco-2 cells than corresponding LMW-MRPs. We conclude that HMW MRPs possess affective antioxidant protection against oxidizable substrates; however, the degree of polymerization of this product, characteristic to the source of monosaccharide used in the reaction, is not a distinguishable factor for this bioactivity. (C) 2004 Elsevier Inc. All rights reserved.
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