期刊
VACCINE
卷 22, 期 29-30, 页码 3968-3975出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2004.03.054
关键词
chelating ISCOMATRIX (TM) adjuvant; ISCOMATRIX (TM) vaccine; HPV; CTL ISCOMATRIX (TM); ISCOM (TM) and ISCOPREP (TM) are trade marks; of ISCOTEC AB a CSL Limited Company
The association of antigen with ISCOMATRIX(TM) adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexa-histidine tags to ISCOMATRIX(TM) adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIX(TM) adjuvant, the denaturant can be removed. Further, we show enhanced CTL responses with a protein-associated chelating ISCOMATRIX(TM) vaccine compared to a non-associated ISCOMATRIX(TM) vaccine. (C) 2004 Elsevier Ltd. All rights reserved.
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