期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 138, 期 1-2, 页码 123-132出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2004.03.021
关键词
dopamine; 5-hydroxytryptamine; norepinephrine; cocaine; RP-HPLC; tandem mass spectrometry; microdialysis
A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialy sates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively. (C) 2004 Elsevier B.V. All rights reserved.
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