期刊
MOLECULAR AND CELLULAR PROBES
卷 18, 期 5, 页码 307-312出版社
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2004.04.004
关键词
real-time PCR; gltA; genospecie; Bartonella spp; endocarditis; CSD
A Real-time PCR for the identification and differentiation of Bartonella spp. based on the detection of mutations in an internal region of the gltA gene by thermal analysis was developed. The assay included a simultaneous detection of the amplicons by direct hybridization of LCRed and fluorescein-labelled probes coupled with melting curve analysis by the use of fluorescence resonance energy transfer technology. The protocol allowed establishing genospecie identity in less than I h without a need for restriction enzyme digestion or gel electrophoresis. The method is simple, reproducible, rapid, specific and potentially transferable to clinical samples. (C) 2004 Elsevier Ltd. All rights reserved.
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