期刊
GENOMICS
卷 84, 期 4, 页码 745-756出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2004.06.001
关键词
histones; H2B; gene structure; gene expression; histone evolution; telomere; testis
Testis-specific histories are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histories by prolamines to epigenetic control of gene transcription. Several testis historic variants were,characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (H2BFWT) located at Xq22.2, which encodes a highly divergent H2B variant. The H2BFWT gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that H2BFWT evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged H2BFWT targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, H2BFWT may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1]. (C) 2004 Elsevier Inc. All rights reserved.
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