期刊
JOURNAL OF PHARMACEUTICAL SCIENCES
卷 93, 期 10, 页码 2624-2634出版社
ELSEVIER SCIENCE INC
DOI: 10.1002/jps.20078
关键词
biodegradable polymers; controlled release/delivery; initial release; poly(lactide-co-glycolide) (PLG) microspheres; particle size; proteins; recombinant human growth hormone (rhGH); spray-freeze drying
Protein microencapsulation in biodegradable polymers is a promising route to provide for sustained release. One important characteristic in this regard is the size of the particles encapsulated within the microspheres. In this investigation, we have employed spray-freeze drying to generate particles for encapsulation, and examined the effect of various atomization conditions. Conditions were identified resulting in minimization of the particle size for the therapeutic protein recombinant human growth hormone (rhGH). The polymer employed was poly(lactide-co-glycolide) (PLG). The greatest friability for the powder, and hence smallest particle size (e.g., sub-micron), was achieved as the mass flow ratio of atomization (air to liquid) was increased. Protein powders over a range of particle sizes were encapsulated in biodegradable microspheres using a cryogenic, non-aqueous process. The initial release (both in vitro and in vivo) from these batches was found to decrease with decreasing encapsulated protein particle size; these findings are consistent with the percolation theory. Hence, judicious selection of process variables to reduce the particle size of rhGH is one strategy that can be used to minimize initial release of the microencapsulated protein. (C) 2004 Wiley-Liss, Inc.
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