期刊
JOURNAL OF VIROLOGICAL METHODS
卷 121, 期 1, 页码 49-55出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2004.05.016
关键词
TSWV; IC/RT-LAMP; detection; chrysanthemum
An immunocapture reverse transcription loop-mediated isothermal amplification (IC/RT-LAMP) was developed for the detection of tomato spotted wilt virus (TSWV) from chrysanthemum. This method enabled sensitive, reproducible and specific detection of TSWV from chrysanthemum plants. In the RT-LAMP method, TSWV genomic RNA could be amplified under isothermal (65degreesC) conditions within 1 h. The resulting amplicons were detected by the measurement or observation of the turbidity of the reaction mixture without gel electrophoresis. IC/RT-LAMP was 100 times more sensitive than IC/RT-PCR. (C) 2004 Elsevier B.V. All rights reserved.
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