期刊
RNA
卷 10, 期 10, 页码 1653-1661出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.7810204
关键词
frameshifting; antisense; morpholino; phosphorothioate; 2 '-O-methyl; recoding
资金
- NIGMS NIH HHS [R01 GM048152, GM48152] Funding Source: Medline
- NINDS NIH HHS [R01 NS043264, R01 NS43264] Funding Source: Medline
Evidence is presented that morpholino, 2'-O-methyl, phosphorothioate, and RNA antisense oligonucleotides can direct site-specific -1 translational frameshifting when annealed to mRNA downstream from sequences where the P- and A-site tRNAs are both capable of re-pairing with -1 frame codons. The efficiency of ribosomes shifting into the new frame can be as high as 40%, determined by the sequence of the frameshift site, as well as the location, sequence composition, and modification of the antisense oligonucleotide. These results demonstrate that a perfect duplex formed by complementary oligonucleotides is sufficient to induce high level -1 frameshifting. The implications for the mechanism of action of natural programmed translational frameshift stimulators are discussed.
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