期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1050, 期 2, 页码 211-216出版社
ELSEVIER
DOI: 10.1016/j.chroma.2004.08.049
关键词
oligosaccharide; capillary electrophoresis; 3-aminobenzoic acid; 8-aminopyrene-1,3,6-trisulfonate; antibody pharmaceutical
Carbohydrate chains in glycoprotein pharmaceuticals have important roles for the expression of their biological activities. Therefore, development of an assessment method for the carbohydrate chains is an important parameter for quality control of glycoprotein pharmaceuticals such as newly developed therapeutic antibodies. In this report, we applied capillary electrophoresis with laser-induced fluorescence detection to the analysis of carbohydrate chains after releasing with glycoamidase followed by derivatization with 3-aminobenzoic acid. We found that four major oligosaccharides present in antibody pharmaceuticals were successfully separated with good resolution. The present method showed good precision in both migration times and relative peak areas, and gave comparable accuracy with that using a derivatization method with 8-aminopyrene-1,3,6-trisulfonate. (C) 2004 Elsevier B.V. All rights reserved.
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