期刊
LEUKEMIA
卷 18, 期 10, 页码 1693-1698出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.leu.2403464
关键词
ALK; tyrosine kinase receptor; anaplastic large-cell lymphoma; small-cell variant; translocation (2;5)
Anaplastic large-cell lymphoma (ALCL) is a distinct biological and cytogenetic entity with a broad spectrum of morphological features ( common type, small-cell variant and lymphohistiocytic variant). Few cell lines of ALCL are available and they all originate from primary tumors demonstrating the common type morphology (ie large-sized lymphoma cells). We established a new ALCL cell line ( COST) from the peripheral blood of a patient with a small-cell variant of ALCL, at diagnosis. Cells growing in vitro and in SCID mice consisted of two populations, that is, small- and large-sized cells as seen in the patient's tumor. Both large and small malignant cells were positive for CD43/MT1 T-cell associated antigen, perforin, granzyme B and TIA-1, but negative for CD2, CD3, CD5, CD7, CD4 and CD8 antigens. Standard cytogenetic studies as well as multiplex FISH confirmed the presence of the canonical t(2; 5)(p23; q35) translocation, but also revealed additional numerical and structural abnormalities. The COST cell line is the first ALCL small- cell variant cell line, and thus provides a potentially useful tool for further functional and molecular studies that should improve our understanding of the small- cell variant of ALCL, which is more frequently complicated by a leukemic phase.
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