期刊
NATURE METHODS
卷 1, 期 1, 页码 19-26出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth709
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资金
- NIGMS NIH HHS [GM58272, GM 46995] Funding Source: Medline
In eukaryotes, chromatin is the natural form of DNA in the nucleus. For hundreds of millions of years, DNA-binding factors have evolved with chromatin. It is therefore more desirable to study the molecular mechanisms of DNA-directed processes with chromatin than with naked DNA templates. To this end, it is necessary to reconstitute DNA and histones into chromatin. Fortunately, there are a variety of methods by which a nonspecialist can prepare chromatin of high quality. Here, we describe strategies and techniques for the reconstitution of chromatin in vitro.
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