期刊
JOURNAL OF ENVIRONMENTAL MANAGEMENT
卷 73, 期 1, 页码 71-79出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jenvman.2004.06.001
关键词
bacterial source tracking; microbial source tracking; E coli; fecal bacteria; water quality; ribotyping; antibiotic resistance analysis (ARA); pulse-field gel electrophoresis (PFGE) d; denaturing-gradient gel electrophoresis (DGGE); repetitive DNA sequences (Rep-PCR); host-specific 16S rDNA
Many molecular and biochemical methods and techniques are being developed to track sources of bacteria in water and food. Currently, there is no standard method proposed for source tracking. This manuscript is a critical evaluation of the various methods used in watersheds, and highlights some of the advantages and disadvantages of each method. Making a decision on a single or combination of methods to use under a particular situation will depend on a number of factors including: question(s) to be answered, scale of identification (broad scale versus specific species identification), available expertise, cost of analysis, turnaround time, and access to facilities. This manuscript reviews several source tracking methodologies which are in current use for source tracking fecal bacteria in the environment including: ribotyping, pulse-field gel electrophoresis, denaturing-gradient gel electrophoresis, repetitive DNA sequences (Rep-PCR), host-specific 16S rDNA genetic markers, and antibiotic resistance analysis. (C) 2004 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据