Reduced antioxidant status in obese children with multimetabolic syndrome

BACKGROUND: In our previous study, the negative correlation found between plasma insulin levels and plasma alpha-tocopherol concentrations suggested that decreased antioxidant vitamin levels and reduced antioxidant capacity might be a characteristic feature of obese children with multimetabolic syndrome (MMS). OBJECTIVE: To investigate lipid-soluble antioxidant vitamin levels and total antioxidant status (TAS) in obese children with and without MMS and in controls. SUBJECTS: In total, 16 control children (age: 16.2+/-1.1 y, BMI: 20.7+/-1.9 kg/m(2), body fat (BF): 25.6+/-5.7%; mean+/-s.d.), 15 obese children (age: 13.4+/-2.1 y, BMI: 34.2+/-3.1 kg/m(2), BF: 36.9+/-5.8%,) and 17 obese children without MMS (age: 14.4+/-2.3 y, BMI: 30.4+/-6.2 kg/m(2), BF: 36.3+/-5.8%) were included in the study. METHODS: Body composition was determined by anthropometric methods. Vitamin analysis was carried out by high-performance liquid chromatography and TAS of the plasma was measured with commercially available kits. Plasma glucose, lipids and insulin were measured by standard laboratory methods. RESULTS: Plasma alpha-tocopherol and beta-carotene levels corrected for plasma lipids (cholesterol + triglyceride) were significantly (P<0.05) lower in obese children with MMS (2.4 (3.1) mu mol/mmol and 12.3 (24.0) pmol/mmol, respectively, median (range from the first to the third quartile)), than in the obese without MMS (3.7 (0.9) mu mol/mmol and 48.2 (27.7) pmol/mmol) and in the control group (3.8 (0.7) mu mol/mmol and 86.6 (44.5) pmol/mmol). Plasma TAS values of the MMS group (1.2 (0.4) mmol/l) were also significantly (P<0.05) reduced as compared to obese children without MMS (1.62 (0.14) mmol/l) and to controls (1.58 (0.21) mmol/l). CONCLUSION: Obese children with MMS are prone to oxidative stress. Further investigations are necessary to determine if these children may benefit from vitamin E and beta-carotene supplementation.