期刊
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
卷 9, 期 7, 页码 828-838出版社
SPRINGER
DOI: 10.1007/s00775-004-0581-9
关键词
cluster assembly; iron-sulfur proteins; IscA protein; scaffold proteins; SufA protein
IscA/SufA proteins belong to complex protein machineries which are involved in iron-sulfur cluster biosynthesis. They are defined as scaffold proteins from which preassembled clusters are transferred to target apoproteins. The experiments described here demonstrate that the transfer reaction proceeds in two observable steps: a first fast one leading to a protein-protein complex between the cluster donor (SufA/IscA) and the acceptor (biotin synthase), and a slow one consisting of cluster transfer leading to the apoform of the scaffold protein and the holoform of the target protein. Mutation of cysteines in the acceptor protein specifically inhibits the second step of the reaction, showing that these cysteines are involved in the cluster transfer mechanism but not in complex formation. No cluster transfer from IscA to IscU, another scaffold of the isc operon, could be observed, whereas IscU was shown to be an efficient cluster source for cluster assembly in IscA. Implications of these results are discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据