期刊
ARTHROPOD STRUCTURE & DEVELOPMENT
卷 33, 期 4, 页码 431-442出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.asd.2004.06.006
关键词
polytrophic meroistic ovary; honeybee; germline stem cells; actin, nuclear tubulin; BrdU
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [99/00719-6] Funding Source: FAPESP
Neither the overall differences in ovariole number nor the caste-specifically modulated expression of vitellogenin can fully explain the striking caste differences in honey bee reproduction, in particular the mechanisms that block oogenesis in virgin queens and in workers kept in the presence of a queen. For this reason we investigated the initial stages of oogenesis in queens in relation to mating status and in workers exposed to different social conditions. A striking feature in ovarioles of both castes was a considerably elongated terminal filament which consisted not only of normal terminal filament cells but also contained apparently undifferentiated cells that were tentatively considered as stem cells. BrdU incorporation was detected in the upper germarium, as well as in the terminal filament. Cytoskeleton analysis by TRITC-phalloidin labeling for F-actin, and immuncifluorescence detection for -Wbulin did not reveal structural differences in the early oogenesis steps between queens and queenless workers. In contrast, queenright workers showed signs of a disorganized microtubule and microfilament system that could explain the histological evidence for progressive cell death observed in the germaria. In addition to cytoplasmic tubulin we also detected marked intranuclear foci indicating the presence of nuclear beta(II)-tubulin. (C) 2004 Elsevier Ltd. All rights reserved.
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