期刊
JOURNAL OF VIROLOGICAL METHODS
卷 121, 期 1, 页码 79-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2004.06.004
关键词
DNA sensing; HBV; gold nanoparticles; silver staining; gene chip
A novel hepatitis B virus (HBV) gene detection chip has been developed. The HBV-specific probes immobilized on glass slides were hybridized with polymerase chain reaction (PCR) products of different serum samples. The hybridization signal can be easily visualized upon a sandwich assay with nanoparticle amplification. The analytical performance (e.g., specificity, sensitivity, and accuracy) of this method has been evaluated. The chip-based detection method possesses a greater sensitivity and a better reproducibility than some of the conventional immunological or molecular biological methods (e.g., enzyme-linked immunosorbent assay, ELISA) and is simple, cost-effective, and highly selective. (C) 2604 Elsevier B.V. All rights reserved.
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