期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 279, 期 40, 页码 41942-41949出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M407821200
关键词
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资金
- NIGMS NIH HHS [R37 GM034478, R01 GM034478, GM58600, GM34478] Funding Source: Medline
The structure of the proton-pumping vacuolar ATPase (V-ATPase) from bovine brain clathrin coated vesicles was analyzed by electron microscopy and single molecule image analysis. A three-dimensional structural model of the complex was calculated by the angular reconstitution method at a resolution of 27 Angstrom. Overall, the appearance of the V-0 and V-1 domains in the three-dimensional model of the intact bovine V-ATPase resembles the models of the isolated bovine V0 and yeast V1 domains determined previously (Wilkens, S., and Forgac, M. ( 2001) J. Biol. Chem. 276, 44064 - 44068; Zhang, Z., Charsky, C., Kane, P. M., and Wilkens, S. ( 2003) J. Biol. Chem. 278, 47299 - 47306). To determine the binding position of subunit H in the V-ATPase, electron microscopy and cysteine-mediated photochemical cross-linking were used. Difference maps calculated from projection images of intact bovine V-ATPase and a V-ATPase preparation in which the two H subunit isoforms were removed by treatment with cystine revealed less protein density at the bottom of the V-1 in the subunit H-depleted enzyme, suggesting that subunit H isoforms bind at the interface of the V-1 and V-0 domains. A comparison of three-dimensional models calculated for intact and subunit H-depleted enzyme indicated that at least one of the subunit H isoforms, although poorly resolved in the three-dimensional electron density, binds near the putative N-terminal domain of the a subunit of the V-0. For photochemical cross-linking, unique cysteine residues were introduced into the yeast V-ATPase B subunit at sites that were localized based on molecular modeling using the crystal structure of the mitochondrial F-1 domain. Cross-linking was performed using the photoactivatable sulfhydryl reagent 4-(N-maleimido) benzophenone. Cross-linking to subunit H was observed from two sites on subunit B (E494 and T501) predicted to be located on the outer surface of the subunit closest to the membrane. Results from both electron microscopy and cross-linking analysis thus place subunit H near the interface of the V-1 and V-0 domains and suggest a close structural similarity between the V-ATPases of yeast and mammals.
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