期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 126, 期 39, 页码 12298-12305出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja046628h
关键词
-
The combination of high metal selectivity of DNAzymes with the strong distance-dependent optical properties of metallic nanoparticles has presented considerable opportunities for designing colorimetric sensors for metal ions. We previously communicated a design for a colorimetric lead sensor based on the assembly of gold nanoparticles by a Pb2+-dependent DNAzyme. However, heating to 50 degreesC followed by a cooling process of similar to2 h was required to observe the color change. Herein we report a new improved design that allows fast (<10 min) detection of Pb2+ at ambient temperature. This improvement of sensor performance is a result of detailed studies of the DNAzyme and nanoparticles, which identified tail-to-tail nanoparticle alignment, and large (42 nm diameter) nanoparticle size as the major determining factors in allowing fast color changes. The optimal conditions for other factors such as temperature (35 degreesC) and concentrations of the DNAzyme (2 muM), its substrate (3 nM), and NaCl (300 mM) have also been determined. These results demonstrate that fundamental understanding of the DNAzyme biochemistry and nanoparticle science can lead to dramatically improved colorimetric sensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据