ZnS-nanocrystals/polypyrrole nanocomposite film based immunosensor

We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (alpha CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (alpha CRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (alpha CRP-Ag) were delineated. The immunosensor exhibited an impedance response to aCRP-Ag concentration in a linear range from 10 ng to 10 mu g mL(-1). (C) 2012 American Institute of Physics. [doi:10.1063/1.3681580]