期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1051, 期 1-2, 页码 237-245出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.08.039
关键词
metallothionein isoforms; cyclodextrin; acrylamide-coated capillaries; capillary zone electrophoresis
Capillary zone electrophoresis (CZE) with cyclodextrin (CD) in the polyacrylamide-coated capillary was used to study metallothionein (MT) forms in the horse kidney preparation produced commercially by Sigma. It is known that CDs form complexes with hydrophobic amino acids. The results show that the presence of CD improves the separability of the various MT forms, including the MT-IA and the MT-IB forms, metallothionein aggregates, as well as the so far unidentified a and b forms. This was true both below and above the isoelectric points (p/s), although the migration times were somewhat longer at increasing CD concentrations for runs at constant voltage than with constant current. (C) 2004 Elsevier B.V. All rights reserved.
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