期刊
BIOINFORMATICS
卷 20, 期 15, 页码 2471-2472出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/bth254
关键词
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PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.
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