期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 41, 页码 9027-9034出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5399-04.2004
关键词
adhesion; imaging; spinal; synaptogenesis; Rohon-Beard; in vivo
N-cadherin is a prominent component of developing and mature synapses, yet very little is known about its trafficking within neurons. To investigate N-cadherin dynamics in developing axons, we used in vivo two-photon time-lapse microscopy of N-cadherin - green fluorescent protein (Ncad - GFP), which was expressed in Rohon - Beard neurons of the embryonic zebrafish spinal cord. Ncad - GFP was present as either stable accumulations or highly mobile transport packets. The mobile transport packets were of two types: tubulovesicular structures that moved preferentially in the anterograde direction and discrete - punctate structures that exhibited bidirectional movement. Stable puncta of Ncad - GFP accumulated in the wake of the growth cone with a time course. Colocalization of Ncad - GFP puncta with synaptic markers suggests that N-cadherin is a very early component of nascent synapses. Expression of deletion mutants revealed a potential role of the extracellular domain in appropriate N-cadherin trafficking and targeting. These results are the first to characterize the trafficking of a synaptic cell-adhesion molecule in developing axons in vivo. In addition, we have begun to investigate the cell biology of N-cadherin trafficking and targeting in the context of an intact vertebrate embryo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据