期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 41, 页码 9059-9066出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1645-04.2004
关键词
channel; deafness; Drosophila; hair cell; hearing; mechanosensory
资金
- NIDCD NIH HHS [DC02780, R01 DC002780] Funding Source: Medline
- NIGMS NIH HHS [R01 GM027318, GM27318] Funding Source: Medline
Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein - IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation.
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